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MB Sample ID: SA270929
Local Sample ID: | CRS6 |
Subject ID: | SU002806 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP002812 |
Sampleprep Summary: | To precipitate proteins, we combined 200 μl SERUM with 800 μl of methanol, incubated at -20 for 30 min, then centrifuged at 18000 rpm for 10 min. Subsequently, 850 μl of supernatant was transferred to a new tube and evaporated to dryness in a Thermo Savant SpeedVac. Then 200 μl of resuspension solvent methanol was added, compounds were dissolved by vortexing for 5 min and samples were clarified by centrifuged at 18000 rpm for 10 min. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -20℃ |