Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA273733

Local Sample ID:	A549-GAA-1
Subject ID:	SU002824
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP002830
Sampleprep Summary:	The collected cells were thawed on ice, then added 500 μM pre-cooled extractant (80% methanol aqueous solution), and whirl for 2 min. Freeze the mixture for 5 min in liquid nitrogen after remove ice for 5 min, it will be whirled for 2 min, circulate this at 3 times. Centrifuge the mixture again with 15000 rpm/min at 4 ℃ for 20 min. Finally take the supernatant into the sample bottle for LC-MS/MS analysis.

Sampleprep ID:

SP002830

Sampleprep Summary:

The collected cells were thawed on ice, then added 500 μM pre-cooled extractant (80% methanol aqueous solution), and whirl for 2 min. Freeze the mixture for 5 min in liquid nitrogen after remove ice for 5 min, it will be whirled for 2 min, circulate this at 3 times. Centrifuge the mixture again with 15000 rpm/min at 4 ℃ for 20 min. Finally take the supernatant into the sample bottle for LC-MS/MS analysis.