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MB Sample ID: SA274840
Local Sample ID: | P_210 |
Subject ID: | SU002838 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002844 |
Sampleprep Summary: | Serum lipids were extracted using a modified Folch method 26. Briefly, 10 µl serum supplemented with a mixture of internal standards were extracted using 1600 μl of acidified methanol:0.05N HCl (1:1 v/v) and 800 μl chloroform in the presence of an antioxidant, butylated hydroxytoluene. The lower organic phase was dried and lipids were reconstituted into 40µl of LC-MS compatible solvent and were quantified by LC-MS/MS. |