Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA274998

Local Sample ID:	P_156
Subject ID:	SU002838
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002844
Sampleprep Summary:	Serum lipids were extracted using a modified Folch method 26. Briefly, 10 µl serum supplemented with a mixture of internal standards were extracted using 1600 μl of acidified methanol:0.05N HCl (1:1 v/v) and 800 μl chloroform in the presence of an antioxidant, butylated hydroxytoluene. The lower organic phase was dried and lipids were reconstituted into 40µl of LC-MS compatible solvent and were quantified by LC-MS/MS.

Sampleprep ID:

SP002844

Sampleprep Summary:

Serum lipids were extracted using a modified Folch method 26. Briefly, 10 µl serum supplemented with a mixture of internal standards were extracted using 1600 μl of acidified methanol:0.05N HCl (1:1 v/v) and 800 μl chloroform in the presence of an antioxidant, butylated hydroxytoluene. The lower organic phase was dried and lipids were reconstituted into 40µl of LC-MS compatible solvent and were quantified by LC-MS/MS.