Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA288699

Local Sample ID:	RJ_30DG1
Subject ID:	SU002843
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

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Sample Preparation:

Sampleprep ID:	SP002849
Sampleprep Summary:	Frozen LV tissue samples (~1-5mg) were weighed into Precellys tubes (Stretton Scientific Ltd., Derbyshire, UK), and an exact volume of extraction solution (50% methanol, 30% acetonitrile and 20% water) was added to obtain 40 mg specimen per ml of extraction solution, permitting comparisons between experimental conditions for the same metabolite. The samples were subsequently lysed after the addition of 3 ceramic beads using a Precellys 24 tissue homogenizer (Bertin Corp, Rockville, MD 20850, USA. 5500 r.p.m for 15 seconds × 2) and finally centrifuged (16,162 × g for 10 min at 4 °C). The supernatant was transferred into glass vials (Microsolv Technology Corp., Leland, NC 28451, USA) and stored at −80 °C until LC–MS analysis.

Sampleprep ID:

SP002849

Sampleprep Summary:

Frozen LV tissue samples (~1-5mg) were weighed into Precellys tubes (Stretton Scientific Ltd., Derbyshire, UK), and an exact volume of extraction solution (50% methanol, 30% acetonitrile and 20% water) was added to obtain 40 mg specimen per ml of extraction solution, permitting comparisons between experimental conditions for the same metabolite. The samples were subsequently lysed after the addition of 3 ceramic beads using a Precellys 24 tissue homogenizer (Bertin Corp, Rockville, MD 20850, USA. 5500 r.p.m for 15 seconds × 2) and finally centrifuged (16,162 × g for 10 min at 4 °C). The supernatant was transferred into glass vials (Microsolv Technology Corp., Leland, NC 28451, USA) and stored at −80 °C until LC–MS analysis.