Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA295621

Local Sample ID:	Ctrl4
Subject ID:	SU002881
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP002887
Sampleprep Summary:	Fresh tissue samples were frozen in liquid nitrogen. After thawing on ice, 10 mg tissue was homogenized in -80°C precooled labeled-solution (MeOH: H2O=4:1, v/v) for 30 s, 4 cycles, and placed at -80°C for 30 minutes. In ice water bath, ultrasonic wave was used for 10 min and centrifuged at 14000 rpm. The precipitated protein was dissolved in no labeled-liquid (MeOH: H2O=4:1), vortexed and centrifuged. After derivatization with N-methyl-N-(trimethylsilyl)tri-fluoroacetamide for 30 min, the supernatant was detected by GC-MS. Metabolites were identified by means of KEGG Metabolic pathways.

Sampleprep ID:

SP002887

Sampleprep Summary:

Fresh tissue samples were frozen in liquid nitrogen. After thawing on ice, 10 mg tissue was homogenized in -80°C precooled labeled-solution (MeOH: H2O=4:1, v/v) for 30 s, 4 cycles, and placed at -80°C for 30 minutes. In ice water bath, ultrasonic wave was used for 10 min and centrifuged at 14000 rpm. The precipitated protein was dissolved in no labeled-liquid (MeOH: H2O=4:1), vortexed and centrifuged. After derivatization with N-methyl-N-(trimethylsilyl)tri-fluoroacetamide for 30 min, the supernatant was detected by GC-MS. Metabolites were identified by means of KEGG Metabolic pathways.