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MB Sample ID: SA297542
Local Sample ID: | 20-0029_166 |
Subject ID: | SU002886 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
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Sample Preparation:
Sampleprep ID: | SP002892 |
Sampleprep Summary: | For metabolite extraction, 0.5 mL of chilled extraction buffer (2:5:2 ratio of ddH2O: methanol: chloroform containing 5µM ribitol as internal standard) was added into each root sample and vortex for 20 secs. Metal beads in the tubes were removed. Sample tubes were shaken on a rotary shaker for 30 mins at 4°C. The samples were then centrifuged at 20, 000g for 5 mins at 4°C. After centrifugation, 0.5 ml of supernatant was carefully aspirated and transferred to a clean 1.5 ml microcentrifuge tube. Samples were stored at-80°C. For GC-MS analysis 30µl of sample were dried by vacuum centrifugation in glass inlet tubes. Dried samples were derivatized according to (Gu et al. 2012 DOI:10.1039/c2ib00109h) and measured as described (Shim et al. 2019 DOI:10.3389/fpls.2019.01726) |