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MB Sample ID: SA299132
| Local Sample ID: | 180821_Poo_Day1and2Poo_Half5.mzML |
| Subject ID: | SU002896 |
| Subject Type: | Other organism |
| Subject Species: | Natural mixed marine microbial community |
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Sample Preparation:
| Sampleprep ID: | SP002902 |
| Sampleprep Summary: | Polar metabolites were extracted using a modified Bligh−Dyer extraction using 1:1 methanol/water and dichloromethane. A methodological blank was extracted and analyzed alongside. Isotope-labeled internal standards were added both before and after the extraction for all samples, blanks, and pooled samples. To evaluate the effect of obscuring variation due to different matrix strengths and analytical drift, pooled samples were run at both full and half concentration (diluted with water) at least three times during the run. This study ran the eddy center samples separately from the eddy transect samples but they were processed together. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Bligh-Dyer |
| Extract Storage: | -80℃ |
