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MB Sample ID: SA305841
| Local Sample ID: | 8 |
| Subject ID: | SU002936 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
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Sample Preparation:
| Sampleprep ID: | SP002942 |
| Sampleprep Summary: | Metabolomics assays for primary metabolomics, biogenic amines, and lipidomics were performed using protein precipitation extraction with UPLC-MS using modified previously published methods [6]. Briefly, 25 µL of plasma were added to 1.5 mL tubes before the addition of 10 µL of 1 µM internal standard solution, followed by 750 µL chilled methanol. Samples were then vortexed 30 seconds prior to being centrifuged at 15,000 x G for 10 min. The supernatant was transferred to 1.5 mL high performance liquid chromatography (HPLC) amber glass vials, dried by centrifugal vacuum evaporation, and reconstituted in 100 µL 3:1 acetonitrile: methanol solution with CUDA solution. The reconstituted solution was vortexed 30 seconds and placed on ice for 10 minutes. The solution was then centrifuged at 10,000 x G for 3 minutes after being transferred to microfilter tubes. The supernatant was then transferred to a HPLC vial to be analyzed using the UPLC-MS. |
| Sampleprep Protocol Filename: | La_Frano_Lab_Methods_Doc_VA_v9[2].pdf |
