Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA309377

Local Sample ID:	A_31
Subject ID:	SU002974
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002980
Sampleprep Summary:	For fecal samples, 60mg of the sample was mixed with 600μL of 80% iced methanol, which included 0.001mg/mL 4-chloro-DL-phenylalanine as an internal standard. Zirconia crushing beads were added to the mixture, and the samples were disrupted using a vibratory crusher operating at 60 hertz. Subsequently, the samples were processed by ultrasonication in an ice bath for 10 minutes and left to stand at -20℃ for 30 minutes. The supernatants were collected by centrifugation at 20,000 g under 4 C for 10 minutes and then mixed with three-fold volumes of distilled water. After ultrasonication for 3 minutes to ensure even mixing, the samples were placed in a -20℃ environment for 2 hours. Finally, the supernatants obtained from centrifugation were transferred to sample vials for metabolomics analysis

Sampleprep ID:

SP002980

Sampleprep Summary:

For fecal samples, 60mg of the sample was mixed with 600μL of 80% iced methanol, which included 0.001mg/mL 4-chloro-DL-phenylalanine as an internal standard. Zirconia crushing beads were added to the mixture, and the samples were disrupted using a vibratory crusher operating at 60 hertz. Subsequently, the samples were processed by ultrasonication in an ice bath for 10 minutes and left to stand at -20℃ for 30 minutes. The supernatants were collected by centrifugation at 20,000 g under 4 C for 10 minutes and then mixed with three-fold volumes of distilled water. After ultrasonication for 3 minutes to ensure even mixing, the samples were placed in a -20℃ environment for 2 hours. Finally, the supernatants obtained from centrifugation were transferred to sample vials for metabolomics analysis