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MB Sample ID: SA313431
| Local Sample ID: | brain-WT-E18-C-04 |
| Subject ID: | SU002983 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP002989 |
| Sampleprep Summary: | Fetal tissue extraction: Following infusion, mice were euthanized and blood was collected via heart puncture. Fetal tissues (placenta, brain, liver, and heart) were dissected in ice-cold sterile PBS. Immediately after dissection, weight was recorded and fetal tissue was placed in a pre-filled bead mill tube containing metal beads and 500 µL of methanol:water (80:20) solution kept cold on dry ice. Fetal tissues were homogenized using a Fisherbrand™ Bead Mill Homogenizer. Samples were spun twice at >17,000 g (4 °C) to remove precipitated cell material (protein/DNA). Supernatants were collected, transferred to a clean tube, and evaporated using a Nitrogen evaporator (Organomation). Evaporated samples were stored at -80 °C. Pellets containing protein/DNA were dried on a heat block (55 °C) and stored at -80 °C. Serum extraction: Collected blood was centrifuged at 5,000g to collect serum. Serum was snap frozen in liquid nitrogen and stored until extraction. For metabolite extraction 5 µL serum was mixed with 500 µL 100% MeOH (-80 °C). Samples were centrifuged for 10 min at >17,000 g (4 °C) and 450 µL of each sample evaporated using a Nitrogen evaporator (Organomation). Evaporated samples were stored at -80 °C. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | -80℃ |
