Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA314479

Local Sample ID:	10
Subject ID:	SU002989
Subject Type:	Mammal
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP002995
Sampleprep Summary:	Soluble metabolites were extracted directly from snap-frozen cell pellets using cold acetonitrile/water (80/20, vol/vol) at a dilution of 10,000 cells/ul solvent. Samples were vortexed and incubated at -80C overnight to precipitate proteins and subsequently thawed, vortexed, and centrifuged at 18,000 x g for 30 min at 4C to pellet debris. The supernatants were transferred to new vials and analyzed by high-performance liquid chromatography (HPLC) and high-resolution mass spectrometry and tandem mass spectrometry (MS/MS).

Sampleprep ID:

SP002995

Sampleprep Summary:

Soluble metabolites were extracted directly from snap-frozen cell pellets using cold acetonitrile/water (80/20, vol/vol) at a dilution of 10,000 cells/ul solvent. Samples were vortexed and incubated at -80C overnight to precipitate proteins and subsequently thawed, vortexed, and centrifuged at 18,000 x g for 30 min at 4C to pellet debris. The supernatants were transferred to new vials and analyzed by high-performance liquid chromatography (HPLC) and high-resolution mass spectrometry and tandem mass spectrometry (MS/MS).