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MB Sample ID: SA314480
Local Sample ID: | 11 |
Subject ID: | SU002989 |
Subject Type: | Mammal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002995 |
Sampleprep Summary: | Soluble metabolites were extracted directly from snap-frozen cell pellets using cold acetonitrile/water (80/20, vol/vol) at a dilution of 10,000 cells/ul solvent. Samples were vortexed and incubated at -80C overnight to precipitate proteins and subsequently thawed, vortexed, and centrifuged at 18,000 x g for 30 min at 4C to pellet debris. The supernatants were transferred to new vials and analyzed by high-performance liquid chromatography (HPLC) and high-resolution mass spectrometry and tandem mass spectrometry (MS/MS). |