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MB Sample ID: SA315434
| Local Sample ID: | D-Gly/0_1-pos |
| Subject ID: | SU003003 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Genotype Strain: | BL21(DE3) |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003009 |
| Sampleprep Summary: | Lipids were extracted from E coli cell pellets following the Matyash protocol with slight variations described in the manuscript supplemental. Cell lysis was performed with bead beating or ultrasonication. Extraction solvents were in the ratio 10:3:25 MTBE:methanol:water. See publication for more detail. |
| Sampleprep Protocol Filename: | Deuterated_Lipids_Methods_Summary.pdf |
| Extraction Method: | Matyash (MTBE) varient |
| Sample Resuspension: | 8:23:69 butanol:isopropanol:water |
