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MB Sample ID: SA316419
Local Sample ID: | SGBS_ctrl_d9_1 |
Subject ID: | SU003027 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP003033 |
Sampleprep Summary: | Intracellular metabolites were extracted using a 1:1:1 methanol:water:chloroform extraction protocol. Briefly, culture media was removed and cells were rinsed with icecold 0.9 % sodium chloride solution. The rinsing solution was removed and the cells metabolism was quenched by adding equal volumes of methanol, followed by icecold deionized water. Cells were scraped off the culture plate and combined chloroform. Samples were vortexed and kept on a shaker at 4°C for 20 min at 1400 rpm. Cell debris was removed by centrifugation. A fixed volume of the polar upper phase was transferred to new tubes and dried to completeness. |