Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA317859

Local Sample ID:	s_Con_5
Subject ID:	SU003037
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Not applicable
Human Race:	Pacific asian

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003043
Sampleprep Summary:	~40 mg of feces was used and lipids were extracted by Bligh and Dyer method after adding internal standard solutions (50 μl of 100 nM C18 ceramide-d7 and C18:1 Sphingomyelin-d9 and 200 μl of 1 μM 1,3-19:0-d5). Organic solutions containing the lipids were dried using a vacuum centrifuge and stored at –20°C until LC-MS/MS analysis. The dried matter was reconstituted with methanol and injected into the LC-MS/MS system.

Sampleprep ID:

SP003043

Sampleprep Summary:

~40 mg of feces was used and lipids were extracted by Bligh and Dyer method after adding internal standard solutions (50 μl of 100 nM C18 ceramide-d7 and C18:1 Sphingomyelin-d9 and 200 μl of 1 μM 1,3-19:0-d5). Organic solutions containing the lipids were dried using a vacuum centrifuge and stored at –20°C until LC-MS/MS analysis. The dried matter was reconstituted with methanol and injected into the LC-MS/MS system.