Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA317890

Local Sample ID:	sample_12
Subject ID:	SU003038
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57BL/6J
Age Or Age Range:	10-week
Gender:	Male

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Subject
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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003044
Sampleprep Summary:	Mouse livers were dissected out and snap-frozen in liquid nitrogen. Liver samples were cryo-homogenized on dry ice and 30 mg of homogenized tissue powders were weighed and extracted with 600 μL ice-cold acetonitrile/methanol/water (4/4/2) for 30 min. After centrifugation at 20,000 g for 15 min, supernatant was transferred to a new tube and dried by a Speedvac vacuum concentrator at 30°C. Dried samples were stored at -80°C and subjected to LC-MS analysis within 24 hours.

Sampleprep ID:

SP003044

Sampleprep Summary:

Mouse livers were dissected out and snap-frozen in liquid nitrogen. Liver samples were cryo-homogenized on dry ice and 30 mg of homogenized tissue powders were weighed and extracted with 600 μL ice-cold acetonitrile/methanol/water (4/4/2) for 30 min. After centrifugation at 20,000 g for 15 min, supernatant was transferred to a new tube and dried by a Speedvac vacuum concentrator at 30°C. Dried samples were stored at -80°C and subjected to LC-MS analysis within 24 hours.