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MB Sample ID: SA322346
Local Sample ID: | POS_EV2_1 |
Subject ID: | SU003072 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP003078 |
Sampleprep Summary: | 1.5–2×106 cells per sample were treated with ice-cold methanol (80% v/v) and they were snap frozen via submergence into liquid Nitrogen for 30 seconds. Subsequently, they were placed on dry ice and allowed to thaw. This step was repeated three times with 10 second vortex-mixing between cycles. At the end, the samples were centrifuged at 11,500 g for 10 min at 4 °C, and the supernatants were collected, lyophilized overnight (~14 h) and stored at −80 °C. |