Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA322359

Local Sample ID:	NEG_SH4_1
Subject ID:	SU003072
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Female

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003078
Sampleprep Summary:	1.5–2×106 cells per sample were treated with ice-cold methanol (80% v/v) and they were snap frozen via submergence into liquid Nitrogen for 30 seconds. Subsequently, they were placed on dry ice and allowed to thaw. This step was repeated three times with 10 second vortex-mixing between cycles. At the end, the samples were centrifuged at 11,500 g for 10 min at 4 °C, and the supernatants were collected, lyophilized overnight (~14 h) and stored at −80 °C.

Sampleprep ID:

SP003078

Sampleprep Summary:

1.5–2×106 cells per sample were treated with ice-cold methanol (80% v/v) and they were snap frozen via submergence into liquid Nitrogen for 30 seconds. Subsequently, they were placed on dry ice and allowed to thaw. This step was repeated three times with 10 second vortex-mixing between cycles. At the end, the samples were centrifuged at 11,500 g for 10 min at 4 °C, and the supernatants were collected, lyophilized overnight (~14 h) and stored at −80 °C.