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MB Sample ID: SA326850
Local Sample ID: | EF_eWAT_neg_3 |
Subject ID: | SU003118 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 12 weeks |
Weight Or Weight Range: | 25-30g |
Gender: | Male |
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Sample Preparation:
Sampleprep ID: | SP003124 |
Sampleprep Summary: | Animals were sacrificed immediately by cervical dislocation. Tissues were subjected to centrifugation (10 min, 800 g, 4°C) following placement in a 20 μm nylon mesh filter (EMD Millipore). Metabolites were extracted by adding extraction buffer at a ratio of 1:100 interstitial fluid to methanol. Samples were then centrifuged twice (5 min, 10,000 g, 4°C) and supernatant was collected. |