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MB Sample ID: SA326856
Local Sample ID: | BAT_chow_neg_4 |
Subject ID: | SU003119 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 12 weeks |
Weight Or Weight Range: | 25-30g |
Gender: | Male |
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Sample Preparation:
Sampleprep ID: | SP003125 |
Sampleprep Summary: | Animals were sacrificed immediately by cervical dislocation and tissues were rapidly extracted and immediately snap frozen in liquid nitrogen for metabolite profiling. For metabolite extraction, tissues were weighed and then homogenized with extraction buffer which consisted of 80% methanol containing D₈-Phenylalanine internal standards at a 40:1 volume to wet weight ratio. Samples were centrifuged at 16,000 x g at 4 °C for 15 min. Finally, 50 μL supernatant was transferred to the glass insert for LC-MS detection. |