Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA326858

Local Sample ID:	BAT_HFD_neg_5
Subject ID:	SU003119
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57BL/6J
Age Or Age Range:	12 weeks
Weight Or Weight Range:	25-30g
Gender:	Male

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP003125
Sampleprep Summary:	Animals were sacrificed immediately by cervical dislocation and tissues were rapidly extracted and immediately snap frozen in liquid nitrogen for metabolite profiling. For metabolite extraction, tissues were weighed and then homogenized with extraction buffer which consisted of 80% methanol containing D₈-Phenylalanine internal standards at a 40:1 volume to wet weight ratio. Samples were centrifuged at 16,000 x g at 4 °C for 15 min. Finally, 50 μL supernatant was transferred to the glass insert for LC-MS detection.

Sampleprep ID:

SP003125

Sampleprep Summary:

Animals were sacrificed immediately by cervical dislocation and tissues were rapidly extracted and immediately snap frozen in liquid nitrogen for metabolite profiling. For metabolite extraction, tissues were weighed and then homogenized with extraction buffer which consisted of 80% methanol containing D₈-Phenylalanine internal standards at a 40:1 volume to wet weight ratio. Samples were centrifuged at 16,000 x g at 4 °C for 15 min. Finally, 50 μL supernatant was transferred to the glass insert for LC-MS detection.