Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA329858

Local Sample ID:	POS_10
Subject ID:	SU003152
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Age Or Age Range:	12 weeks
Weight Or Weight Range:	25 g
Gender:	Male

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Sample Preparation:

Sampleprep ID:	SP003158
Sampleprep Summary:	Mouse liver (100 mg) was homogenized in 500 µl PBS using FisherBrand BeadMill. Homogenates were spiked with 10 µl SPLASH-lipidomics internal standard mix (Avanti Polar Lipids/CRODA), transferred to glass tubes and extracted using the method of Bligh and Dyer. Lipid extracts were evaporated under a gentle stream of nitrogen gas and resuspended in 100 µl of HPLC-grade methanol and chloroform (9:1 ratio). Discovery lipidomics were performed using a Vanquish ultrahigh performance liquid chromatography (UHPLC) system interfaced to a Q Exactive HF quadrupole/orbitrap mass spectrometer (Thermo Fisher Scientific) operating in data-dependent mode. Lipid extracts (5 µl) were injected in both positive and negative ESI modes. Pooled QCs were injected to assess the performance of the LC and MS instruments at the beginning, middle and at the end of each sequence.

Sampleprep ID:

SP003158

Sampleprep Summary:

Mouse liver (100 mg) was homogenized in 500 µl PBS using FisherBrand BeadMill. Homogenates were spiked with 10 µl SPLASH-lipidomics internal standard mix (Avanti Polar Lipids/CRODA), transferred to glass tubes and extracted using the method of Bligh and Dyer. Lipid extracts were evaporated under a gentle stream of nitrogen gas and resuspended in 100 µl of HPLC-grade methanol and chloroform (9:1 ratio). Discovery lipidomics were performed using a Vanquish ultrahigh performance liquid chromatography (UHPLC) system interfaced to a Q Exactive HF quadrupole/orbitrap mass spectrometer (Thermo Fisher Scientific) operating in data-dependent mode. Lipid extracts (5 µl) were injected in both positive and negative ESI modes. Pooled QCs were injected to assess the performance of the LC and MS instruments at the beginning, middle and at the end of each sequence.