Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA333265

Local Sample ID:	01_028
Subject ID:	SU003208
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116
Weight Or Weight Range:	400g
Gender:	Male and female
Animal Animal Supplier:	Charles River
Animal Housing:	Double Housed
Animal Light Cycle:	Reverse Light Cycle
Species Group:	Sprague Dawley

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Sample Preparation:

Sampleprep ID:	SP003214
Sampleprep Summary:	Experimental batch 1 and 2 aliquoted tissue samples were thawed simultane-ously on ice prior to addition of solvent (IPA and Splash II Lipidomix in (1:3 v/v)) to sep-arate lipids and small non-polar metabolites. LC-MS grade water was used to prepare sample blanks, and pooled quality control (QC) samples were prepared from 5 µL ali-quoted supernatant of all samples in the study. The brain and solvent (1:4 w/v), and beads were placed in a Tissuelyser II for 8 min and centrifuged at 16000 g for 7 min. The supernatant was collected for LC-MS. Pooled quality control samples were formed from combining 6 µL aliquots of all brain sample extracts. Sample blanks were prepared with the same procedure except instead of a brain sample, 50 µL of LC-MS grade water was used.

Sampleprep ID:

SP003214

Sampleprep Summary:

Experimental batch 1 and 2 aliquoted tissue samples were thawed simultane-ously on ice prior to addition of solvent (IPA and Splash II Lipidomix in (1:3 v/v)) to sep-arate lipids and small non-polar metabolites. LC-MS grade water was used to prepare sample blanks, and pooled quality control (QC) samples were prepared from 5 µL ali-quoted supernatant of all samples in the study. The brain and solvent (1:4 w/v), and beads were placed in a Tissuelyser II for 8 min and centrifuged at 16000 g for 7 min. The supernatant was collected for LC-MS. Pooled quality control samples were formed from combining 6 µL aliquots of all brain sample extracts. Sample blanks were prepared with the same procedure except instead of a brain sample, 50 µL of LC-MS grade water was used.