Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA333638

Local Sample ID:	50
Subject ID:	SU003220
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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NMR

Sample Preparation:

Sampleprep ID:	SP003226
Sampleprep Summary:	Plasma was thawed at room temperature for 15 min before transferring 150 µl to pre-washed ultrafiltration tubes (Amicon Ultra 0.5 ml, 3k MWCO, Merck Millipore) which were spun at 14000 x g, at 4°C for 90 min. 90 µl of each filtrate was mixed with 90 µl buffer (75 mM sodium phosphate pH 7.4, 0.1% w/v sodium azide, 3.66 mM 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSPd4), in 20% v/v D2O) and transferred to 3 mm SampleJet NMR-tubes.

Sampleprep ID:

SP003226

Sampleprep Summary:

Plasma was thawed at room temperature for 15 min before transferring 150 µl to pre-washed ultrafiltration tubes (Amicon Ultra 0.5 ml, 3k MWCO, Merck Millipore) which were spun at 14000 x g, at 4°C for 90 min. 90 µl of each filtrate was mixed with 90 µl buffer (75 mM sodium phosphate pH 7.4, 0.1% w/v sodium azide, 3.66 mM 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSPd4), in 20% v/v D2O) and transferred to 3 mm SampleJet NMR-tubes.