Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA338936

Local Sample ID:	HetCD_mixpos
Subject ID:	SU003244
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003251
Sampleprep Summary:	In brief, 1 ml of 90% isopropanol was added to 15 mg of homogenized heart tissue. Samples were vortexed for 2 min, sonicated for 1 min using a probe sonicator and centrifuged at 15,000 g for 10 min. The supernatant was collected and dried down using a SpeedVac. The dried samples were reconstituted using acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS analysis.

Sampleprep ID:

SP003251

Sampleprep Summary:

In brief, 1 ml of 90% isopropanol was added to 15 mg of homogenized heart tissue. Samples were vortexed for 2 min, sonicated for 1 min using a probe sonicator and centrifuged at 15,000 g for 10 min. The supernatant was collected and dried down using a SpeedVac. The dried samples were reconstituted using acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS analysis.