Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA340621

Local Sample ID:	Sample7
Subject ID:	SU003259
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP003266
Sampleprep Summary:	The lipids extraction was performed strictly following the SOP established based on a modified Folch liquid-liquid extraction protocol. Briefly, an aliquot of 4.5 μL of each sample was vortexed with 1.5 μL of internal standard solution and methanol, followed by adding dichloromethane and vortexing for another 20 s. A clean-up step was performed with water and 10 seconds of vortex. Samples were allowed to equilibrate at room temperature for 10 min and centrifuged at 16,000 g for 10 min at 4°C. An aliquot of the organic layer was evaporated to dryness with a nitrogen blowdown evaporator. The residue was re-suspended in 4.5 μL of NovaMT MixB, vortexed for 1 min, and diluted with 40.5 μL of NovaMT MixA

Sampleprep ID:

SP003266

Sampleprep Summary:

The lipids extraction was performed strictly following the SOP established based on a modified Folch liquid-liquid extraction protocol. Briefly, an aliquot of 4.5 μL of each sample was vortexed with 1.5 μL of internal standard solution and methanol, followed by adding dichloromethane and vortexing for another 20 s. A clean-up step was performed with water and 10 seconds of vortex. Samples were allowed to equilibrate at room temperature for 10 min and centrifuged at 16,000 g for 10 min at 4°C. An aliquot of the organic layer was evaporated to dryness with a nitrogen blowdown evaporator. The residue was re-suspended in 4.5 μL of NovaMT MixB, vortexed for 1 min, and diluted with 40.5 μL of NovaMT MixA