Summary of Study ST002459

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001586. The data can be accessed directly via it's Project DOI: 10.21228/M8PQ70 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002459
Study Title	Comparison of metabolite profiles from matched whole blood microsamplers, whole blood dried blood spots, and plasma.
Study Summary	Venous blood was collected from 54 adult female participants from the PRISM cohort. Whole blood and venous blood was aliquoted. Untargeted metabolomics was performed on whole blood collected on Mitra microsamplers (VAMS, 10 uL), whole blood dried blood spots (DBS, 5-mm punch), and 10 uL of plasma
Institute	Icahn School of Medicine at Mount Sinai
Last Name	Petrick
First Name	Lauren
Address	1428 Madison Avenue, Atran Building
Email	lauren.petrick@mssm.edu
Phone	9739976141
Submit Date	2023-01-22
Total Subjects	54
Num Females	54
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-01-22
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002555
Sampleprep Summary:	In allocated randomized order, 5-mm DBS punches, 10 uL blood microsamplers, and 10 uL plasma aliquots were thawed on ice and placed into microcentrifuge tubes. 100 uL of water was added, vortexed, and 5 uL reserved for hemoglobin analysis. 400 uL of acetonitrile containing internal standards was added, vortexed, stored at -20C overnight to precipitate proteins, and centrifuged the next day. Two aliquots of the supernatant were evaporated to dryness and stored in -80°C until analysis with LC-HRMS. From all samples, 40 uL of the supernatant were combined to generate a pooled QC sample. Following the same extraction protocol a 5-mm blank punch from adjacent Whatman paper and a blank microsampler pen were extracted from 5 participants, respectively. Similarly, plasma was replaced with 10 uL of water and extracted. The respective extracts were pooled to generate a matrix plasma (matrix P), matrix DBS (matrix D), and matrix VAM (matrix V).

Sampleprep ID:

SP002555

Sampleprep Summary:

In allocated randomized order, 5-mm DBS punches, 10 uL blood microsamplers, and 10 uL plasma aliquots were thawed on ice and placed into microcentrifuge tubes. 100 uL of water was added, vortexed, and 5 uL reserved for hemoglobin analysis. 400 uL of acetonitrile containing internal standards was added, vortexed, stored at -20C overnight to precipitate proteins, and centrifuged the next day. Two aliquots of the supernatant were evaporated to dryness and stored in -80°C until analysis with LC-HRMS. From all samples, 40 uL of the supernatant were combined to generate a pooled QC sample. Following the same extraction protocol a 5-mm blank punch from adjacent Whatman paper and a blank microsampler pen were extracted from 5 participants, respectively. Similarly, plasma was replaced with 10 uL of water and extracted. The respective extracts were pooled to generate a matrix plasma (matrix P), matrix DBS (matrix D), and matrix VAM (matrix V).