Summary of Study ST002519
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001620. The data can be accessed directly via it's Project DOI: 10.21228/M89B04 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002519 |
Study Title | Time course 3: Growth of Eggerthella lenta in defined media with some samples receiving 13C6 stable isotope labeled arginine (intracellular samples) |
Study Type | Untargeted LC-MS |
Study Summary | This dataset contains untargeted metabolomics analysis of supernatants from Eggerthella lenta grown in defined EDM1 media. One set of samples grew in EDM1 containing 13C6 stable isotope labeled arginine. Samples were collected at a subset of time points for extraction of intracellular metabolites. |
Institute | University of California, San Francisco |
Last Name | Noecker |
First Name | Cecilia |
Address | 513 Parnassus Ave HSW1501, San Francisco, CA 94143 |
cecilia.noecker@ucsf.edu | |
Phone | 415-502-3264 |
Submit Date | 2023-03-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML, raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-09 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002625 |
Sampleprep Summary: | Prior to analysis, intracellular samples were dried at room temperature via Centrivap Benchtop Concentrator (Labconco Corp.). Samples were re-suspended in 60 μL of a chilled solution of 1:1 methanol and acetonitrile, with 24% water at -20oC containing the internal standards CUDA and VAL-TYR-VAL each at 60 ng/mL. Samples were centrifuged at 4°C, 4,122 rcf for 5 minutes and the supernatant transferred to a vial and immediately capped for LC-MS analysis. |