Summary of Study ST003167
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001970. The data can be accessed directly via it's Project DOI: 10.21228/M8314P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003167 |
Study Title | Metabolomics of Human CD19-BBz CD8 CAR-T cells with low and high MCJ expression |
Study Summary | The goal of these experiments are to characterize the metabolic profile of the human CD8 CD19-BBz CAR-T cells from donors with high or low MCJ expression after 3 expansions (6 days) with IL-2 (100IU/ml). |
Institute | University of Colorado School of Medicine |
Laboratory | Laboratory of Angelo D'Alessandro in collaboration with Mercedes Rincon |
Last Name | Cendali |
First Name | Francesca |
Address | 13199 East Montview Boulevard, Aurora, CO, 80045, USA |
francesca.cendali@cuanschutz.edu | |
Phone | 3037246131 |
Submit Date | 2024-04-11 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-05-02 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003293 |
Sampleprep Summary: | CAR-T cells were isolated as described above either from in vitro culture or from bone marrow harvested in an in vivo study. The cells were washed in PBS and frozen at -80C until the assay is ready to run. Metabolites from cells were extracted at 2x106 cells/ml at 4°C (30 min) in the presence of 5:3:2 MeOH:MeCN:water (v/v/v). The samples were spun down and the resulting supernatant was transferred to new tubes and dried under a vacuum. The resulting residue was reconstituted in 0.1% formic acid at a 3x concentration, then analyzed on a Thermo Vanquish UHPLC coupled to a Thermo Q Exactive MS as previously described in detail. |