Summary of Study ST000232

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000182. The data can be accessed directly via it's Project DOI: 10.21228/M8M888 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000232
Study Title	Untargeted metabolomic analysis of the small intestinal content of malnourished mice
Study Type	Metabolomics
Study Summary	A total of 8 samples from 6 week old, female C57BL/6 mice, treated for 3 weeks with a malnourished diet or a control-fed isocaloric diet. Samples were taken from the small intestinal fecal content at the terminus of the ileum.
Institute	University of Victoria
Department	The Uvic Proteomics and Metabolomics Innovation Centre
Last Name	Borchers
First Name	Christoph
Address	#3101-4464 Markham St Victoria, BC Canada, V8Z 7X8
Email	christoph@proteincentre.com
Submit Date	2015-06-01
Num Groups	2
Total Subjects	8
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Uploaded File Size	1.1 G
Analysis Type Detail	LC-MS
Release Date	2015-08-14
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000255
Sampleprep Summary:	Metabolite Extraction. Mouse small intestine digesta samples (luminal contents) were collected, weighed and homogenized in water followed by addition of acetonitrile for metabolite extraction. After vortex mixing, 30 second sonication, and centrifugation, the clear supernatants were collected and dried under a gentle nitrogen gas flow. The dried metabolite residues were dissolved in 40% acetonitrile containing 0.01% formic acid, 4 ?L per mg of the raw material. After 15 sec vortex and 30 sec sonication, each sample was further diluted 1:2 with water. Twelve-?L aliquots were injected for untargeted metabolic fingerprinting by ultrahigh-performance liquid chromatography ? Fourier transform mass spectrometry (UPLC-FTMS).

Sampleprep ID:

SP000255

Sampleprep Summary:

Metabolite Extraction. Mouse small intestine digesta samples (luminal contents) were collected, weighed and homogenized in water followed by addition of acetonitrile for metabolite extraction. After vortex mixing, 30 second sonication, and centrifugation, the clear supernatants were collected and dried under a gentle nitrogen gas flow. The dried metabolite residues were dissolved in 40% acetonitrile containing 0.01% formic acid, 4 ?L per mg of the raw material. After 15 sec vortex and 30 sec sonication, each sample was further diluted 1:2 with water. Twelve-?L aliquots were injected for untargeted metabolic fingerprinting by ultrahigh-performance liquid chromatography ? Fourier transform mass spectrometry (UPLC-FTMS).