Summary of Study ST000382
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000299. The data can be accessed directly via it's Project DOI: 10.21228/M8D60P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000382 |
Study Title | Urinary Metabolites in IC/PBS Diagnosis (part II) |
Study Type | Disease |
Study Summary | This West Coast Metabolomics Center sponsored pilot grant goal is to identify and validate interstitial cystitis/painful bladder syndrome (IC/PBS)-associated urinary metabolites. Our central hypothesis is that IC/PBS-associated metabolites in the urine of IC/PBS patients can segregate patients from control subjects, and that their levels are correlated with clinical symptoms. To test this hypothesis, we will identify IC/PBS-associated metabolites in urine using two independent platforms, GC-MS and quadrupole time-of-flight (Q-TOF) mass spectrometry under the collaboration with UC Davis WCMC scientists. We believe that this study will provide a significant potential clinical impact because results may lead to clinical methods to increase diagnostic accuracy and an improved understanding of the molecular basis of IC/PBS and its relationship to urologic conditions with overlapping symptoms. |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | Health Sciences Drive, Davis, California, 95616, USA |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-04-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000410 |
Sampleprep Summary: | 1. The dry sample is resuspended in 50 uL of Water/acetonitrile 95:5 containing 100 ng/mL of CUDA (12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid) as internal standard, vortexed for 10 sec, sonicated for 5 minutes, then centrifuged for 3 min at 14,000 rcf. 2. The samples are transferred to amber vials with micro-inserts and capped immediately, and stored at 4 degree C until and during analysis |
Sampleprep Protocol Filename: | SP_RP18_Protocol_KimUrine_IG102814.docx |