Summary of Study ST000626
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000456. The data can be accessed directly via it's Project DOI: 10.21228/M83W49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000626 |
Study Title | Non targeted metabolomic analysis of mouse and human bronchoalveolar lavage fluid, Lipid(-) experiment (part II) |
Study Type | Comprehensive profile |
Study Summary | Mouse BALF was collected from C57BL/6 mice (n = 10). Five mice were exposed to ambient air for one day (air control) and five mice were exposed to CS for nine months (smoking). Human BALF was collected from subjects from the COPDGene cohort (n = 5). COPD diagnosis was based on the ratio of forced expiratory volume in 1 second to forced vital capacity (FEV1/FVC). Subjects were 45-70 years old, BMI 27-45, weight 76-125kg, and were categorized as follows: 1 male former smoker without COPD (FEV1/FVC = 0.82), 2 male current smokers without COPD (FEV1/FVC = 0.91 and 0.79), 1 female current smoker with moderate COPD (FEV1/FVC = 0.51), and 1 male current smoker with moderate COPD (FEV1/FVC = 0.64) |
Institute | University of Colorado Denver |
Department | Anschutz Medical Campus, Pharmaceutical Sciences |
Laboratory | Metabolomics and Mass Spectrometry |
Last Name | Reisdorph |
First Name | Nichole |
Address | 12850 East Montview Blvd, Denver, CO 80204 |
nichole.reisdorph@ucdenver.edu | |
Phone | - |
Submit Date | 2017-04-21 |
Num Groups | 2 |
Total Subjects | 36 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2018-12-11 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000656 |
Sampleprep Summary: | 100uL of BALF were prepared using methanol assisted protein precipitation followed by methyl-tert butyl ether (MTBE) liquid-liquid extraction. Both liquid and aqueous fractions were preserved for analysis. The lipid fraction was reconstituted in 200µL of methanol. The lipid fraction was dried down in a SpeedVac at 45°C for reconstitution in 50 µL of 95:5 water:acetonitrile. |