Summary of Study ST000929
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000643. The data can be accessed directly via it's Project DOI: 10.21228/M8JT38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000929 |
| Study Title | Metabolomics Analysis of Aqueous Humour in the Presence of Glaucoma Drainage Devices |
| Study Type | Metabolomics Evaluation of Aqueous Humor Samples from Left (treated) and Right (Control) Eyes of Rabbits. |
| Study Summary | Metabolomic experiments were carried out on aqueous humor samples provided by Ramesh Ayyala. Through this collaboration, we propose to study aqueous humor from control and experimental rabbits to better understand metabolic differences in the aqueous humor samples. |
| Institute | University of North Carolina |
| Department | Nutrition Research Institute |
| Laboratory | ERCMRC |
| Last Name | Sumner |
| First Name | Susan |
| Address | 500 Laureate Way, Kannapolis, NC, 28081, USA |
| susan_sumner@unc.edu | |
| Phone | 7042505066 |
| Submit Date | 2018-02-11 |
| Num Groups | 9 |
| Total Subjects | 56 |
| Num Males | 56 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000975 |
| Sampleprep Summary: | Whole Study Pooled QC Thawed aqueous humor samples on ice. Whole study pooled QC samples were created by combining 10 µL aliquot from each of the study samples (Time Points B, C and D) into a 2 mL Lo-Bind eppendorf tube. The QC pooled sample was then vortexed for 30 seconds. Time Point A Pools Aqueous humor samples were collected from both, right and left, eyes and provided for metabolomics analysis. Aqueous humor from the right eyes from experiment A were combined into a 2 mL Lo-Bind Eppendorf tube to make a right eye pool from experiment A. This same procedure was repeated to make a pool from the left eye aqueous humor from experiment A. The two time point A pools were vortexed for 30 seconds. Sample Preparation To make the study samples, aqueous humor (30 µL) was transferred from their original tubes into 2.0 mL Lo-Bind Eppendorf tubes. Six whole study pooled QC samples of 30 µL each were created along with three-30 µL time point A pool from right eye and three-30 µL time point A pool from left eye aqueous humor. For all samples (study, whole study pooled QC and time point A samples) 400 µL Protein Precipitation Buffer with internal standard (0.025 mg/mL of Tryptophan-d5 in methanol) was added to each tube and vortexed. The samples were then centrifuged at 16,000 rcf for 4 min. A 350 µL aliquot of the supernatant from each sample was transferred into pre-labeled 2.0 mL LoBind eppendorf tube and stored at -80 °C for one hour followed by a drying step on a lyophilizer. The residue was reconstituted in 100 µL of reconstitution buffer (95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 10 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and the supernatants were transferred to autosampler vials for data acquisition. |
| Sampleprep Protocol ID: | Protocol: RTI-RCMRC-LCMS-SA-018 Version: 1.0 |
| Sampleprep Protocol Filename: | 18 AP-Williamson-Aqueous humor-RTI-RCMRC-LCMS-SA-018_v1.0.docx |
| Processing Method: | Protein Precipitation followed by Metabolite Extraction and Lyophilization |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Cold Methanol with Internal Standard |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 95:5 Water:Methanol |
| Sample Spiking: | L-Tryptophan-d5 |
