Summary of Study ST001156
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000775. The data can be accessed directly via it's Project DOI: 10.21228/M8H98P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001156 |
Study Title | Comparative Metabolomic Profiling of Two Contrasting Date Palm Genotypes under Salinity Stress |
Study Summary | Since metabolites are the net products of the central dogma of cellular biology, this study was aimed to decipher salinity tolerance depends on the information encoded by the metabolomic profiles of the salt tolerant ‘Umsila’ and susceptible ‘Zabad’ cultivars when grown under salinity. Changes in the metabolomic profiles of the leaf and root tissues were determined using hydrophilic interaction liquid chromatography (HILIC) and reverse phase liquid (RPLC) mass spectrometry. |
Institute | Sultan Qaboos University |
Last Name | Yaish |
First Name | Mahmoud |
Address | Department of Biology, College of Science, Sultan Qaboos University |
mack.yaish@gmail.com | |
Phone | 968 24146823 |
Submit Date | 2019-03-18 |
Raw Data Available | Yes |
Raw Data File Type(s) | metdb |
Analysis Type Detail | LC-MS |
Release Date | 2020-03-18 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001229 |
Sampleprep Summary: | Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra. |