Summary of Study ST002044

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001291. The data can be accessed directly via it's Project DOI: 10.21228/M8V12R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002044
Study Title	An observational study of cardiovascular patients in India
Study Summary	Serum samples for CVD patients were analyzed by untargeted metabolomics assays to identify prognostic biomarkers.
Institute	Translational Health Science And Technology Institute (THSTI)
Last Name	Kumar
First Name	Yashwant
Address	NCR Biotech Science Cluster 3rd Milestone, Faridabad - Gurgaon Rd, Expressway, Faridabad, Haryana 121001, India
Email	yashubt@gmail.com
Phone	0129-2876300
Submit Date	2021-12-22
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2022-02-08
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002132
Sampleprep Summary:	Serum samples (100µl) should be properly thawed and spin at 5K rpm for one minute, added with 100 % MeOH (300µl, in1:3 ratio) and vortex well (30s), incubated on ice for 10 minutes and then centrifuged at 10K rpm for 10 minutes at 4℃. Equally, transfer the supernatant into two different well-labeled tubes and then speed vacuum dry and stored at -80℃. One set of the tubes is used for the analysis of polar metabolites and the other one is used for the non-polar metabolites. For the reconstitution of polar metabolites, 50% ACN (50 µl) is added into the vacuum dried sample and for nonpolar 15% of MeOH (50 µl) is used. After the addition of reconstitutes, vortex well for 10 min and centrifuged at 10K rpm for 10 minutes at 4℃ and transfer the supernatant (approx. 40-45 µl) into MS vials. Remove 10µl form every sample containing MS vials and pooled together into a 15 ml tube to use as QC. Then, the samples were stored at -80℃ for further analysis.

Sampleprep ID:

SP002132

Sampleprep Summary:

Serum samples (100µl) should be properly thawed and spin at 5K rpm for one minute, added with 100 % MeOH (300µl, in1:3 ratio) and vortex well (30s), incubated on ice for 10 minutes and then centrifuged at 10K rpm for 10 minutes at 4℃. Equally, transfer the supernatant into two different well-labeled tubes and then speed vacuum dry and stored at -80℃. One set of the tubes is used for the analysis of polar metabolites and the other one is used for the non-polar metabolites. For the reconstitution of polar metabolites, 50% ACN (50 µl) is added into the vacuum dried sample and for nonpolar 15% of MeOH (50 µl) is used. After the addition of reconstitutes, vortex well for 10 min and centrifuged at 10K rpm for 10 minutes at 4℃ and transfer the supernatant (approx. 40-45 µl) into MS vials. Remove 10µl form every sample containing MS vials and pooled together into a 15 ml tube to use as QC. Then, the samples were stored at -80℃ for further analysis.