Summary of Study ST002185
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001392. The data can be accessed directly via it's Project DOI: 10.21228/M8SH87 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002185 |
| Study Title | Lipidomic characterization of Jurkat-derived T cell line in which the chaperonin complex CCT has been partially silenced |
| Study Type | Untargeted Lipidomics |
| Study Summary | When the chaperonin complex CCT is partially silenced in Jurkat-derived T cell line J77 E61, we observe changes in the production of exosomes and in their composition. Thus, to explore the bases of these alterations and find possible new mechanisms of exosome biosynthesis regulation we characterized the lipidome content in cells where the chaperonin complex CCT was partially silent (CCT) and controls (CTRL). We analyzed 5 biological replicates containing 3 × 106 cells using LC-MS in positive and negative polarity mode. For quality control, 5 QCs samples and 4 blanks were also included in the analysis (total 19 samples and 2 groups). |
| Institute | Centro Nacional de Investigaciones Cardiovasculares Carlos III |
| Department | Proteomics and Metabolomics Unit |
| Laboratory | Metabolomics Lab |
| Last Name | Ferrarini |
| First Name | Alessia |
| Address | Calle de Melchor Fernández Almagro, 3, Madrid, Madrid, 28029, Spain |
| aferrarini@cnic.es | |
| Phone | 914 53 12 00 |
| Submit Date | 2022-06-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-06 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002277 |
| Sampleprep Summary: | Five biological replicate containing 3 × 106 cells were collected and stored at -80°C. The cell pellets were thaw on ice and subjected to three freeze–thaw cycles for complete cell disruption and protein precipitation. Briefly, samples were suspended in 250 µL freshly prepared methanol:acetic acid (98:2 v/v) solution, vortex-mixed, placed in liquid nitrogen for 10s and thaw in an ice bath (for 10s) three times. Subsequently samples were centrifuge at 18000g for 20 min. at 10°C. Supernatants were collected and lipids were extracted with methyl-tert-butylether (MTBE) as described (Matyash et al., 2008). 400 µL of organic phase were dried-out in speedvac and resuspended in 50 µL of ACN:H2O (20:80, v:v) just before injection. In order to assess the reproducibility and robusteness of the methodology, quality control samples (QC) were prepared by polling together one CCT and one CTRL sample’s supernatant and following the same extraction procedures. Dried extracted were resuspended in 50 µL of ACN:H2O (20:80, v:v) just before injection. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
