Summary of Study ST002358

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples | Perform analysis on untargeted data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST002358
Study Title	Effect of hypoxia and reoxygenation on the juvenile hooded seal brain lipidome
Study Summary	Brain samples from juvenile hooded seals (Cystophora cristata) were subjected to hypoxia and reoxygenation in vitro and lipid composition was compared.
Institute	University of Hamburg
Last Name	Martens
First Name	Gerrit Alexander
Address	Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Email	gerrit.alexander.martens@uni-hamburg.de
Phone	+49 40 42838-3934
Submit Date	2022-11-23
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	LC-MS
Release Date	2023-04-12
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002453
Sampleprep Summary:	The extraction protocol was performed slightly modified according to the method of Bligh and Dyer (Bligh and Dyer 1959). About 20 mg sample was weighed into a 2.0 ml reaction tube (Eppendorf, Hamburg, Germany). Two steel balls (3.6 mm), 100 µl chloroform and 200 µl methanol were added to the sample. The mixture was homogenized in a ball mill (1 min, 3.1 m/s Bead Ruptor 24, Omni International IM, GA, USA)). Afterwards 200 µl water and 100 µl chloroform were added and again processed in the ball mill (1 min, 3.1 m/s). The homogenized sample was then centrifuged (20 min, 16.000xg, 5 °C, Sigma 3-16PK, Sigma, Osterode, Germany). A quality control sample (QC) was prepared by transferring 30 µl of each sample into a new vial. The organic chloroform phase was directly used for measurement.

Sampleprep ID:

SP002453

Sampleprep Summary:

The extraction protocol was performed slightly modified according to the method of Bligh and Dyer (Bligh and Dyer 1959). About 20 mg sample was weighed into a 2.0 ml reaction tube (Eppendorf, Hamburg, Germany). Two steel balls (3.6 mm), 100 µl chloroform and 200 µl methanol were added to the sample. The mixture was homogenized in a ball mill (1 min, 3.1 m/s Bead Ruptor 24, Omni International IM, GA, USA)). Afterwards 200 µl water and 100 µl chloroform were added and again processed in the ball mill (1 min, 3.1 m/s). The homogenized sample was then centrifuged (20 min, 16.000xg, 5 °C, Sigma 3-16PK, Sigma, Osterode, Germany). A quality control sample (QC) was prepared by transferring 30 µl of each sample into a new vial. The organic chloroform phase was directly used for measurement.