Summary of Study ST002699
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001671. The data can be accessed directly via it's Project DOI: 10.21228/M8Q716 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002699 |
| Study Title | Spatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation |
| Study Summary | KCl timecourse|Neuronal activity creates an intense energy demand that must be met by rapid metabolic responses. To define the metabolic adaptations of the neuron-enriched dentate granule cell (DGC) layer within the native tissue environment, we utilize acute hippocampal brain slices and perform fast metabolite preservation followed by mass spectrometry imaging (MALDI-MSI) to obtain spatially resolved metabolomics and isotope tracing data. We find that membrane depolarization promotes broad metabolic changes, including increased glycolytic activity in DGCs which is accompanied by mobilization of endogenous inosine into pentose phosphates. |
| Institute | Harvard University |
| Last Name | Yellen |
| First Name | Gary |
| Address | 220 Longwood Ave |
| gary_yellen@hms.harvard.edu | |
| Phone | 617-432-0137 |
| Submit Date | 2023-04-04 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | imzML |
| Analysis Type Detail | MALDI-MS |
| Release Date | 2023-08-08 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002809 |
| Sampleprep Summary: | At the specific time chosen for thermal preservation, the slices were flash heated to 80°C for 2 seconds and then cooled to 4°C; the slices were then flash-frozen to -20 °C. The brain slices were stored at -80°C for subsequent thin sectioning in a cryomicrotome. The thin sections were mounted onto conductive glass slides (ITO slides) and sprayed with matrix (1,5-diaminonaphthalene). |
