Summary of Study ST002749
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001712. The data can be accessed directly via it's Project DOI: 10.21228/M8DM8V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002749 |
| Study Title | Bromeliad lipidomics for adaptation to elevation |
| Study Summary | Untargeted analysis on Bromeliades leaf samples. The aim of this study was to evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the different altitudes where they were collected. A lipidomic approach was applied to the samples. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL was used to perform data preprocessing. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on the altitude. Our study grouped the individuals into three major groups: one with individuals from the lower elevations (UBA, RAN, and COR), another with individuals from the highest elevations (PAP and MAR), and another with individuals from mixed localities from intermediate elevations. Higher elevation population showed an increase in very long chain fatty acids and monounsaturated lipids compared to lower elevation as a response to cold higher elevations affecting the leaves' membrane fluidity. In addition, the higher elevation population showed a higher abundance of hexosylceramides and phosphatidylglycerol compared to lower ones. |
| Institute | University of Campinas |
| Department | Chemistry's Institute |
| Laboratory | Laboratory of Bioanalytics and Integrated Omics |
| Last Name | Matos |
| First Name | Taynara |
| Address | Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, Brazil |
| t262827@dac.unicamp.br | |
| Phone | (85)996154192 |
| Submit Date | 2023-06-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-08-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002862 |
| Sampleprep Summary: | Lipid extraction was based on Hummel et al. Method. 60 mL of solvent mixture was prepared with pre-cooled (-20ºC) methanol (MeOH, grade HPLC, LiChrosolv® Reag. Ph. Eur.) and methyl-tert-butyl-ether (MTBE, grade HPLC, purity 99,9%, Sigma-Aldrich) in proportion (1:3 v/v). In a 2 mL tube, 50 mg of macerated sample were added and 1 mL of the solvent mixture. The samples were incubated for 5 min under agitation at 500 rpm at 4 ºC (Microtube Shaking Incubator AccuTherm, Labnet International, Inc.), followed by an ultrasonication (Branson 5800 Ultrasonic Bath, Emerson, Danbury, USA) in ice-cold bath in 10 minutes. After adding 500 μL mixture of water type I:MeOH (3:1 v/v), the samples were vortexed and centrifuged for 5 min at 4 ºC, 10000 rpm (Hettich Zentrifugen Mikro 220R, Tuttlingen, DE). The three phases were separated and dried in a vacuum concentrator (Concentrator Plus, Eppendorf AG, Hamburg, DE), at ambient temperature under vacuum - alcoholic mode, and stored at -80 ºC until the chromatographic analysis. |
| Sampleprep Protocol Filename: | SamplePrep-BromeliadLipidomics.pdf |
