Summary of Study ST002851
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001785. The data can be accessed directly via it's Project DOI: 10.21228/M8043D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002851 |
| Study Title | Metabolic caracterization of liver metastasis organotropism |
| Study Summary | Transcriptomic and metabolomic analyses in animals revealed distinct metabolic adaptations, particularly related to the TCA cycle and OxPhos, specific to liver metastases compared to concurrent lung metastases. This finding was substantiated by analyzing RNA-seq data from a considerable number of patient metastases across various cancer types. Further analysis of exome/RNA-seq data from melanoma patients indicated more frequent PIK3CA mutations, lower transcript levels of PIP4K2C, and enrichment of TCA cycle and OxPhos pathways in liver metastases. |
| Institute | Columbia University |
| Department | Department of Medicine |
| Laboratory | Division of Hematology/Oncology |
| Last Name | Izar |
| First Name | Benjamin |
| Address | Columbia University Irving Medical Center, New York, NY, USA |
| bi2175@cumc.columbia.edu | |
| Phone | 0123456789 |
| Submit Date | 2023-08-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-02 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002969 |
| Sampleprep Summary: | Metabolites were extracted by a two-step liquid extraction adapted from Sellik et al.[1], 50 mg of frozen tissue was used and 500 µL of prechilled methanol (-80 °C) was added. For quantification and data normalization, 25 µL of internal standard solution containing 13C6-L-Arginine, 13C5 L Valine, 13C2-Citric acid, 2H4-Succinic acid and 13C6-Fructose-6-phosphate at a concentration of 100 µM was added. This results in a concentration of 10 µM in the final extract. The sample was homogenized by a tissue slicer and the extract was vortexed for 2 min until no larger cell clusters were visible. Afterward, the sample was sonicated for 2 min in a chilled (0 °C) ultrasonic bath. The cells were kept at -80 °C for 5 min and subsequently thawed. The thawed cells were vortexed and sonicated for 2 min each and centrifuged at 3000 x g for 5 min. The supernatant was collected and 250 µL of water acidified with 0.1 % acetic acid (LC-MS grade) was added. The sample was vortexed and sonicated for 2 min and subject to the same freezing-thawing cycle described before. The samples were centrifuged at 3000 x g for 5 min and the supernatant was collected. The combined supernatant was dried in a vacuum-centrifuge for 45 min. The residue was resuspended in 250 µL acetonitrile/water (50/50; v/v) by sonicating and vortexing for 2 min each. To remove remaining proteins or other cell debris the extracts have been centrifuged at 12,000 rpm for 2 min and filtered using Nanosep 3KDa Omega centrifugal filters (Pall, Port Washington, USA). After the extraction pooled QC samples were generated for the whole sample set as well as for each investigated sample group and analyzed along the study samples to monitor the analytical quality. Ref: [1] (Sellick CA, Hansen R, Stephens GM, Goodacre R, Dickson AJ. Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling. Nat Protoc. 2011 28;6(8):1241-9. doi: 10.1038/nprot.2011.366) |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
