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MB Sample ID: SA001774
Local Sample ID: | Test_1S |
Subject ID: | SU000057 |
Subject Type: | Invertebrate |
Subject Species: | Caenorhabditis elegans |
Taxonomy ID: | 6239 |
Genotype Strain: | N2 |
Age Or Age Range: | Young Adult |
Gender: | Hermaphrodite |
Species Group: | Invertebrate |
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Treatment:
Treatment ID: | TR000058 |
Treatment Summary: | Heat Shock: Two successive synchronous generations of wild-type C. elegans (N2) were grown in S-complete buffer at 22C containing 5% 13C-labeled E. coli. Upon reaching the young adult stage, the population was washed from E. coli using standard procedures and was divided into four replicates. Each replicate was treated with a 30 min heat shock at 33 °C followed by incubation at 22C for 1.5 hours. Control: Two successive synchronous generations of wild-type C. elegans (N2) were grown in S-complete buffer at 22C containing 95% 13C-labeled E. coli. Upon reaching the young adult stage, the population was washed from E. coli using standard procedures. |
Treatment: | Abiotic |
Treatment Dose: | 30C | 22C |
Treatment Doseduration: | 30C for 30 minutes; 1.5 hours at 22 C before harvest | 22C for 30 minutes; 1.5 hours at 22 C before harvest |
Animal Acclimation Duration: | - |