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MB Sample ID: SA011491
Local Sample ID: | CC268_EBSS2 |
Subject ID: | SU000271 |
Subject Type: | Animal cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Animal Animal Supplier: | inhouse breeding |
Cell Biosource Or Supplier: | BM derived B cells |
Cell Strain Details: | wt and EuMyc |
Cell Primary Immortalized: | primary |
Species Group: | Mammal |
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Treatment:
Treatment ID: | TR000282 |
Treatment Summary: | Cells were incubated for 2hrs in EBSS at 37C. |
Cell Media: | 45%DMEM, 45%IMDM, 10%FCS, Q, PS, Sodium Pyruvate, beta mercaptoethanol and EBSS with 10% dialized FBS |
Cell Harvesting: | washed, and pellet flashfroozen in liquid N2 |
Cell Pct Confluence: | 2xE06 per ml |