Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA015130

Local Sample ID:	Syngeneic 4Stool
Subject ID:	SU000358
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Treatment:

Treatment ID:	TR000372
Treatment Summary:	BMTs were performed as previously described {Reddy:2005dc}{Reddy:2008kl}. Briefly, syngeneic (BALB/c ? BALC/b or C57BL/6 ? C57BL/6) and allogeneic (C57BL/6 ? BALB/c or C3H.sw ? C57BL/6) recipients received lethal irradiation. On day -1, BALB/c recipients received a total of 800 cGy of irradiation (split dose separated by 3 hours) and B6 animals received a single dose of 1000 cGy. Donor splenic CD90.2+ T cells were magnetically separated using an autoMACs (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 x 106 – 1 x 106 T cells were transferred to BALB/c recipients and 2 x 106 T cells were transferred to C57BL/6 recipients. 5 x 106 donor whole bone marrow was transferred to all recipients.

Treatment ID:

TR000372

Treatment Summary:

BMTs were performed as previously described {Reddy:2005dc}{Reddy:2008kl}. Briefly, syngeneic (BALB/c ? BALC/b or C57BL/6 ? C57BL/6) and allogeneic (C57BL/6 ? BALB/c or C3H.sw ? C57BL/6) recipients received lethal irradiation. On day -1, BALB/c recipients received a total of 800 cGy of irradiation (split dose separated by 3 hours) and B6 animals received a single dose of 1000 cGy. Donor splenic CD90.2+ T cells were magnetically separated using an autoMACs (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 x 106 – 1 x 106 T cells were transferred to BALB/c recipients and 2 x 106 T cells were transferred to C57BL/6 recipients. 5 x 106 donor whole bone marrow was transferred to all recipients.