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MB Sample ID: SA018758
Local Sample ID: | 140619dlvsa04_1 |
Subject ID: | SU000416 |
Subject Type: | Cells |
Subject Species: | Synechococcus elongatus PCC 7942 |
Taxonomy ID: | 1140 |
Species Group: | Microorganism |
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Treatment:
Treatment ID: | TR000430 |
Treatment Summary: | 2: WT bacteria and KaiC mutant The phenotype associated with loss of the core oscillator protein, KaiC, is distinct from that caused by absence of the circadian output transcriptional regulator, RpaA (regulator of phycobilisome-associated A). Untargeted metabolomics analysis and glycogen kinetics suggest that functional KaiC is important for metabolite partitioning in the morning. Additionally, output from the oscillator functions to inhibit RpaA activity in the morning, and kaiC-null strains expressing a mutant KaiC phosphomimetic, KaiC-pST, in which the oscillator is locked in the most active output state, phenocopies a ΔrpaA strain. KaiC-null strains show indications of oxidative pentose phosphate pathway activation as well as increased abundance of primary metabolites. Inhibitory clock output may serve to allow secondary metabolite biosynthesis in the morning, and some metabolites resulting from these processes may feed back to reinforce clock timing. |
Treatment Protocol Filename: | StudyDesign-SpencerDiamond-10814.pdf |