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MB Sample ID: SA028893
| Local Sample ID: | 528-8 |
| Subject ID: | SU000581 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Gender: | Male |
| Animal Animal Supplier: | KO: bred and housed at the laboratory animal resource unit (LARU) of North Carolina A&T State University; WT: Charles River Laboratories |
| Animal Housing: | group cages of up to 5 mice per cage |
| Animal Light Cycle: | 12:12h light:dark cycle |
| Animal Feed: | rat chow |
| Animal Water: | water ad libitum |
| Species Group: | Mammal |
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Treatment:
| Treatment ID: | TR000595 |
| Treatment Summary: | Type 1 diabetes was induced at age 8 weeks. The mice were fasted for 6 hours prior to being injected with low dose STZ (50 mg/kg body weight) in sodium citrate buffer (10 mmol/L, pH 4.5) daily for 5 days following the protocols described by Tesch and Allen and recommended by the Animal Models of Diabetic Complications Consortium (AMDCC; amdcc.org)1. Control mice were injected with equivalent volumes of the sodium citrate buffer. Diabetes was confirmed by measuring fasting blood glucose levels using a standard glucose meter at 10 days post-STZ injection. Mice with a fasting glucose level ≥280 mg/dL were considered diabetic. STZ-injected mice with a fasting blood glucose of <280 mg/dL (15mmol/L) were culled and eliminated from the study. |
| Treatment Compound: | streptozotocin |
| Treatment Dose: | 50 mg/kg body weight |
| Treatment Doseduration: | daily; 5 days |
| Treatment Vehicle: | sodium citrate |
| Animal Endp Tissue Coll List: | plasma, urine, kidney tissue |
