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MB Sample ID: SA057225
Local Sample ID: | WT3_48h_5mM_1 |
Subject ID: | SU000994 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl6 OT-1 |
Age Or Age Range: | 6-8 weeks |
Animal Animal Supplier: | Breeding at Moffitt for several generations, originally from Jax |
Animal Light Cycle: | 12 hour |
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Treatment:
Treatment ID: | TR001008 |
Treatment Summary: | Splenic CD8+ T cells were isolated from 4 OT-1 mice (WT1, 3, 7, 8) by negative immunomagnetic selection. T cells stimulated with ova-derived peptide (SIINFEKL) that binds with high affinity to the OT-1 transgenic TCR and 10ng/mL IL-2 with and without 5mM DFMO for 48-72 hours. Cell stoarge: 37 degrees, Cell growth container: 24 well plate, cell media: RPMI1640 with glutamine. Added components were: NEAA, Sodium Pyruvate, Fetal Bovine Serum, Fungizone, Gentimycin, Pen/strep. |