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MB Sample ID: SA078049
Local Sample ID: | KO3_3 |
Subject ID: | SU001187 |
Subject Type: | Bacteria |
Subject Species: | Bacteroides thetaiotaomicron VPI-5482 |
Taxonomy ID: | 226186 |
Genotype Strain: | WT (VPI-5482), SPT K.O. (BT_0870) |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | Wild type Bacteroides tethaiotamicron strains and serine palmitoyltransferase (SPT) knockouts |
Subject Comments: | Grown in Minimal Media |
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Treatment:
Treatment ID: | TR001202 |
Treatment Summary: | In-frame deletion of the serine palmitoyl transferase gene (spt) in B. thetaiotaomicron Δtdk was generated using a counter-selectable allelic exchange procedure (Koropatkin et al., 2008). |
Cell Storage: | -80C |
Cell Growth Container: | anaerobic chamber (Coy Laboratory Products) with an atmosphere of 20% CO2, 5% H2, and 75% N2 at 37°C. |