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MB Sample ID: SA085213

Local Sample ID:7
Subject ID:SU001278
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:53 years
Gender:Female
Cell Biosource Or Supplier:Coriell
Cell Strain Details:GM05372
Cell Primary Immortalized:Epstein-Barr Virus
Cell Passage Number:Six
Cell Counts:15 million

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Treatment:

Treatment ID:TR001293
Treatment Summary:Cells were maintained under normal growth conditions in a humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2. Cells were kept at density between 2.0x105 and 1.0x106 cells/mL to maintain logarithmic growth conditions to promote maximum proliferative activity over the experimental period. All experiments were conducted within six passages following removal from cryopreservation. On the day of experimentation, K562 cells were seeded at a density of 4.0x105 cells/mL and exposed to 1000 nM MTX or D-PBS (i.e. vehicle) for 24 hours under normal growth conditions.
Treatment Compound:Methotrexate
Treatment Route:In Media
Treatment Dose:1000 nM
Treatment Doseduration:24 hours
Treatment Vehicle:Dulbecco's Phosphate Buffered Saline (without Calcium/Magnesium)
Cell Storage:Liquid Nitrogen
Cell Growth Container:T-175 Flask
Cell Growth Config:Suspension
Cell Inoc Proc:Seeded at 4.010^5 cells/mL
Cell Media:RPMI 1640 medium (catalog no. 61870-127, ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (catalog no. S11150, Atlanta Biologicals, Lawrenceville, GA) along with 100 units/mL penicillin and 1,000 units/mL streptomycin (catalog no. 15140122, ThermoFisher Scientific, Waltham, MA)
Cell Envir Cond:humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2
Cell Harvesting:Counted and a volume containing 15x10^6 cells were collected, washed, flash frozen in liquid nitrogen
Cell Pct Confluence:NA
Cell Media Lastchanged:24 hours prior to collection
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