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MB Sample ID: SA085223
Local Sample ID: | 17 |
Subject ID: | SU001278 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 53 years |
Gender: | Female |
Cell Biosource Or Supplier: | Coriell |
Cell Strain Details: | GM05372 |
Cell Primary Immortalized: | Epstein-Barr Virus |
Cell Passage Number: | Six |
Cell Counts: | 15 million |
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Treatment:
Treatment ID: | TR001293 |
Treatment Summary: | Cells were maintained under normal growth conditions in a humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2. Cells were kept at density between 2.0x105 and 1.0x106 cells/mL to maintain logarithmic growth conditions to promote maximum proliferative activity over the experimental period. All experiments were conducted within six passages following removal from cryopreservation. On the day of experimentation, K562 cells were seeded at a density of 4.0x105 cells/mL and exposed to 1000 nM MTX or D-PBS (i.e. vehicle) for 24 hours under normal growth conditions. |
Treatment Compound: | Methotrexate |
Treatment Route: | In Media |
Treatment Dose: | 1000 nM |
Treatment Doseduration: | 24 hours |
Treatment Vehicle: | Dulbecco's Phosphate Buffered Saline (without Calcium/Magnesium) |
Cell Storage: | Liquid Nitrogen |
Cell Growth Container: | T-175 Flask |
Cell Growth Config: | Suspension |
Cell Inoc Proc: | Seeded at 4.010^5 cells/mL |
Cell Media: | RPMI 1640 medium (catalog no. 61870-127, ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (catalog no. S11150, Atlanta Biologicals, Lawrenceville, GA) along with 100 units/mL penicillin and 1,000 units/mL streptomycin (catalog no. 15140122, ThermoFisher Scientific, Waltham, MA) |
Cell Envir Cond: | humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2 |
Cell Harvesting: | Counted and a volume containing 15x10^6 cells were collected, washed, flash frozen in liquid nitrogen |
Cell Pct Confluence: | NA |
Cell Media Lastchanged: | 24 hours prior to collection |